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Identification of a novel splicing isoform of murine CGI‐58
Author(s) -
Yang Xingyuan,
Lu Xin,
Liu Jun
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.12.058
Subject(s) - gene isoform , adipose triglyceride lipase , exon , perilipin , alternative splicing , rna splicing , lipid droplet , biology , complementary dna , gene , microbiology and biotechnology , biochemistry , adipose tissue , lipase , adipocyte , enzyme , rna
The comparative gene identification‐58 (CGI‐58) gene, mutations of which are linked to Chanarin‐Dorfman syndrome, encodes a protein of the α/β hydrolase domain subfamily. We report here a new alternative splicing isoform of the murine CGI‐58 gene, termed mCGI‐58S. Sequence comparison indicates the lack of second and third exons in this cDNA variant. While the full‐length protein displayed perilipin‐dependent localization to lipid droplets, mCGI‐58S showed a predominant cytoplasmic staining when expressed in cells. mCGI‐58S was incapable of activating adipose triglyceride lipase but retained the capacity to acylate lysophosphatidic acid. Overexpression of mCGI‐58S failed to promote lipid droplet turnover and loss of intracellular triacylglycerols. These results suggest that this splicing event may be involved in the regulation of lipid homeostasis.