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Panky , a novel photoreceptor‐specific ankyrin repeat protein, is a transcriptional cofactor that suppresses CRX‐regulated photoreceptor genes
Author(s) -
Sanuki Rikako,
Omori Yoshihiro,
Koike Chieko,
Sato Shigeru,
Furukawa Takahisa
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.12.030
Subject(s) - ankyrin repeat , ankyrin , cofactor , gene , microbiology and biotechnology , chemistry , biology , biochemistry , enzyme
Neuronal gene transcription is regulated by both transcriptional activators and repressors. While the roles of transactivators in retinal photoreceptor development have been well characterized, the roles of repressors have been poorly understood. We isolated Panky/Ankrd33, a gene encoding an ankyrin repeat‐containing protein. Panky‐A was specifically expressed in retinal photoreceptors and the pineal gland, and its expression was directly up‐regulated by the CRX transcription factor. Subcellular localization of PANKY‐A was observed in the nucleus and cytoplasm. Additionally, transactivation analysis suggested that PANKY‐A is a transcriptional cofactor that suppresses CRX‐activated photoreceptor genes. Furthermore, we found by an electrophoretic mobility shift assay that PANKY inhibited the DNA‐binding activity of CRX.