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The yeast aquaglyceroporin Fps1p is a bidirectional arsenite channel
Author(s) -
Maciaszczyk-Dziubinska Ewa,
Migdal Iwona,
Migocka Magdalena,
Bocer Tomasz,
Wysocki Robert
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.12.027
Subject(s) - arsenite , arsenate , saccharomyces cerevisiae , arsenic , yeast , efflux , downregulation and upregulation , detoxification (alternative medicine) , biochemistry , biology , transporter , microbiology and biotechnology , chemistry , gene , medicine , alternative medicine , organic chemistry , pathology
The stress‐activated kinase Hog1p mediates arsenic tolerance by decreasing arsenite influx through the aquaglyceroporin Fps1p in Saccharomyces cerevisiae . Unexpectedly, we found that overexpression of FPS1 increased arsenite tolerance suggesting a physiological role of Fps1p in arsenic detoxification. Consistently, during arsenite treatment transcription of FPS1 gene was strongly upregulated, while Fps1p was not degraded and remained localized to the plasma membrane. Moreover, deletion of FPS1 gene resulted in arsenate sensitivity. Finally, transport experiments revealed that Fps1p in concert with the arsenite transporter Acr3p mediates arsenite efflux.