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Mitochondria‐targeted antioxidant SkQR1 selectively protects MDR (Pgp 170)‐negative cells against oxidative stress
Author(s) -
Fetisova Elena K.,
Avetisyan Armine V.,
Izyumov Denis S.,
Korotetskaya Mariya V.,
Chernyak Boris V.,
Skulachev Vladimir P.
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.12.002
Subject(s) - oxidative stress , mitochondrion , glutathione , chemistry , p glycoprotein , biochemistry , oxidative phosphorylation , antioxidant , multiple drug resistance , biology , microbiology and biotechnology , enzyme , antibiotics
A conjugate of plastoquinone with decylrhodamine 19 (SkQR1) selectively accumulates in mitochondria of normal and tumor cells. SkQR1 protected the cellular pool of reduced glutathione under oxidative stress. Overexpression of P‐glycoprotein (Pgp 170) multidrug resistance pump strongly suppresses accumulation of SkQR1. The inhibitors of Pgp 170 stimulate accumulation of SkQR1 in various cell lines indicating that SkQR1 is a substrate of Pgp 170. The protective effect of SkQR1 against oxidative stress is diminished in the cells overexpressing Pgp 170. It is suggested that mitochondria‐targeted antioxidants could selectively protect normal (Pgp 170‐negative) cells against the toxic effect of anti‐cancer treatments related to oxidative stress.