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Sialic acids acquired by Pseudomonas aeruginosa are involved in reduced complement deposition and siglec mediated host‐cell recognition
Author(s) -
Khatua Biswajit,
Ghoshal Angana,
Bhattacharya Kaushik,
Mandal Chandan,
Saha Bibhuti,
Crocker Paul R.,
Mandal Chitra
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.11.087
Subject(s) - siglec , microbiology and biotechnology , sialic acid , bacteria , pseudomonas aeruginosa , recombinant dna , biology , complement system , host (biology) , immune system , chemistry , biochemistry , immunology , gene , genetics
The opportunism of Pseudomonas aeruginosa (PA) in immunocompromised hosts prompted us to explore the potential role of sialic acids (Sia) in this phenomenon. Culture of PA in the presence of exogenous Sia resulted in linkage‐specific incorporation of Sia which was associated with decreased complement deposition on the bacteria. Sia acquired by PA mediated enhanced binding of bacteria to recombinant‐CHO cells expressing human siglec‐7 or siglec‐9, as well as to human NK‐cells and monocytes naturally expressing these siglecs. Therefore, Sia may be acquired by PA in the host and contribute to bacterial pathogenicity and host‐cell interactions via reduction of complement deposition and siglec‐dependent recognition.