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Yeast acetic acid‐induced programmed cell death can occur without cytochrome c release which requires metacaspase YCA1
Author(s) -
Guaragnella Nicoletta,
Bobba Antonella,
Passarella Salvatore,
Marra Ersilia,
Giannattasio Sergio
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.11.072
Subject(s) - cytochrome c , yeast , programmed cell death , cytochrome , biochemistry , biology , caspase , apoptosis , hydrogen peroxide , microbiology and biotechnology , chemistry , enzyme
To investigate the role of cytochrome c (cyt c ) release in yeast acetic acid‐induced programmed cell death (AA‐PCD), wild type (wt) and cells lacking metacaspase (Δyca1), cytochrome c (Δcyc1,7) and both (Δcyc1,7Δyca1) were compared for AA‐PCD occurrence, hydrogen peroxide (H 2 O 2 ) production and caspase activity. AA‐PCD occurs in Δcyc1,7 and Δcyc1,7Δyca1 cells slower than in wt, but similar to that in Δyca1 cells, in which no cytochrome c release occurs. Both H 2 O 2 production and caspase activation occur in these cells with early and extra‐activation in Δcyc1,7 cells. We conclude that alternative death pathways can be activated in yeast AA‐PCD, one dependent on cyt c release, which requires YCA1, and the other(s) independent on it.