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Singlet oxygen inhibits ATPase and proton translocation activity of the thylakoid ATP synthase CF1CFo
Author(s) -
Buchert Felix,
Forreiter Christoph
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.11.040
Subject(s) - atp synthase , thylakoid , f atpase , atp hydrolysis , adenosine triphosphate , chemiosmosis , photosystem , singlet oxygen , atpase , photosynthesis , v atpase , chemistry , biophysics , photophosphorylation , biochemistry , atp synthase gamma subunit , chromosomal translocation , photosystem ii , biology , chloroplast , oxygen , enzyme , organic chemistry , gene
Singlet oxygen ( 1 O 2 ) produced in plants during photosynthesis has a strong damaging effect not only on both photosystems but also on the whole photosynthetic machinery. This is also applicable for the adenosine triphosphate (ATP) synthase. Here we describe the impact of 1 O 2 generated by the photosensitizer Rose Bengal on the ATP hydrolysis and ATP‐driven proton translocation activity of CF1CFo. Both activities were reduced dramatically within 1 min of exposure. Interestingly, it is shown that oxidized thylakoid ATP synthase is more susceptible to 1 O 2 than CF1CFo in its reduced state, a new insight on the mechanism of 1 O 2 interaction with the γ subunit.