Premium
Processivity of translation in the eukaryote cell: Role of aminoacyl‐tRNA synthetases
Author(s) -
Mirande Marc
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.11.027
Subject(s) - transfer rna , translation (biology) , aminoacylation , biology , t arm , aminoacyl trna synthetase , protein biosynthesis , ribosome , eukaryote , processivity , ribosomal rna , aminoacyl trna , biochemistry , eukaryotic translation , microbiology and biotechnology , rna , enzyme , messenger rna , gene , polymerase , genome
Several lines of evidence led to the conclusion that mammalian ribosomal protein synthesis is a highly organized biological process in vivo. A wealth of data support the concept according to which tRNA aminoacylation, formation of the ternary complex on EF1A and delivery of aminoacyl‐tRNA to the ribosome is a processive mechanism where tRNA is vectorially transferred from one component to another. Polypeptide extensions, referred to as tRBDs (tRNA binding domains), are appended to mammalian and yeast aminoacyl‐tRNA synthetases. The involvement of these domains in the capture of deacylated tRNA and in the sequestration of aminoacylated tRNA, suggests that cycling of tRNA in translation is mediated by the processivity of the consecutive steps. The possible origin of the tRBDs is discussed.