Premium
Shrinking and development of lipid droplets in adipocytes during catecholamine‐induced lipolysis
Author(s) -
Nagayama Masafumi,
Shimizu Kyoko,
Taira Toshio,
Uchida Tsutomu,
Gohara Kazutoshi
Publication year - 2010
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.10.088
Subject(s) - lipolysis , lipid droplet , hormone sensitive lipase , catecholamine , perilipin , chemistry , adipocyte , medicine , endocrinology , fragmentation (computing) , adipose tissue , biology , biochemistry , ecology
Time‐lapse observation of adipocytes during catecholamine‐induced lipolysis clearly shows that shrinking of existing lipid droplets (LDs) occurs in some adipocytes and that small LDs are newly developed in almost all cells. Immunofluorescence imaging reveals that activation and localization of hormone‐sensitive lipase (HSL) on the surface of LDs, which are required for conferring maximal lipolysis, are necessary for the shrinking of the LDs. However, not all adipocytes in which phosphorylated HSL is localized on LDs exhibit shrinking of LDs. The simultaneous shrinking and development of LDs yield apparent fragmentation and dispersion of LDs in adipocytes stimulated with catecholamine.