AKAP 18 α and γ have opposing effects on insulin release in INS‐1E cells

A‐kinase anchoring proteins (AKAPs) are known to compartmentalise protein kinase(s) to discrete cellular locations. Here we show that silencing of AKAP 18 α or γ expression results in decreased or increased glucose‐stimulated insulin secretion in INS‐1E cells. Glucose stimulates AKAP 18 α and inhibits AKAP 18 γ mRNA expressions while palmitate markedly reduces AKAP 18 α expression. Human growth hormone (GH) stimulates AKAP 18 α expression and attenuates palmitate‐induced suppression of AKAP 18 α mRNA level. The roles of AKAP 18 α and γ in mediating insulin release are consistent with their respective regulations by glucose.