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Correlation of mRNA and protein in complex biological samples
Author(s) -
Maier Tobias,
Güell Marc,
Serrano Luis
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.10.036
Subject(s) - messenger rna , ribosome , protein biosynthesis , computational biology , biology , correlation , protein expression , protein turnover , chemistry , biochemistry , rna , gene , geometry , mathematics
The correlation between mRNA and protein abundances in the cell has been reported to be notoriously poor. Recent technological advances in the quantitative analysis of mRNA and protein species in complex samples allow the detailed analysis of this pathway at the center of biological systems. We give an overview of available methods for the identification and quantification of free and ribosome‐bound mRNA, protein abundances and individual protein turnover rates. We review available literature on the correlation of mRNA and protein abundances and discuss biological and technical parameters influencing the correlation of these central biological molecules.