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Large telomerase RNA, telomere length heterogeneity and escape from senescence in Candida glabrata
Author(s) -
Kachouri-Lafond R.,
Dujon B.,
Gilson E.,
Westhof E.,
Fairhead C.,
Teixeira M.T.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.10.034
Subject(s) - telomere , telomerase , telomerase reverse transcriptase , senescence , biology , rna , telomerase rna component , reverse transcriptase , candida glabrata , phenotype , mutant , genetics , microbiology and biotechnology , dna , gene , candida albicans
Telomerase, the key enzyme essential for the maintenance of eukaryotic chromosome ends, contains a reverse transcriptase and an RNA that provides the template for the synthesis of telomeric repeats. Here, we characterize the telomerase subunits in the hemiascomycete yeast Candida glabrata . We propose a secondary structure model for the telomerase RNA that is the largest described to date. Telomerase deletion mutants show a progressive shortening of telomeres and a modest loss of viability. Frequent post‐senescence survivors emerge that possess long telomeric repeat tracts. We suggest that the high telomere length heterogeneity accounts for this distinct senescence phenotype.