Premium
Nitric oxide and dihydrolipoic acid modulate the activity of caspase 3 in HepG2 cells
Author(s) -
Sengupta Rajib,
Billiar Timothy R.,
Atkins James L.,
Kagan Valerian E.,
Stoyanovsky Detcho A.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.10.016
Subject(s) - nitric oxide , chemistry , nitric acid , microbiology and biotechnology , apoptosis , biochemistry , inorganic chemistry , biology , organic chemistry
Herein, we report that dihydrolipoic acid and lipoic acid (LA) plus lipoamide dehydrogenase and NADH denitrosate S ‐nitrosocaspase 3 (CASP‐SNO). In HepG2 cells, S ‐nitroso‐ l ‐cysteine ethyl ester (SNCEE) impeded the activity of caspase 3 (CASP‐SH), while a subsequent incubation of the cells in SNCEE‐free medium resulted in endogenous denitrosation and reactivation of CASP‐SH. The latter process was inhibited in thioredoxin reductase‐deficient HepG2 cells, in which, however, LA markedly reactivated CASP‐SH. The data obtained are discussed with focus on low molecular mass dithiols that mimic the activity of thioredoxin in reactions of protein S ‐denitrosation.