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Two‐dimensional pulsed electron spin resonance characterization of 15 N‐labeled archaeal Rieske‐type ferredoxin
Author(s) -
Iwasaki Toshio,
Samoilova Rimma I.,
Kounosu Asako,
Dikanov Sergei A.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.09.050
Subject(s) - ferredoxin , sulfolobus solfataricus , hyperfine structure , chemistry , electron paramagnetic resonance , electron , resonance (particle physics) , nuclear magnetic resonance , crystallography , physics , atomic physics , archaea , biochemistry , enzyme , gene , quantum mechanics
Two‐dimensional electron spin‐echo envelope modulation (ESEEM) analysis of the uniformly 15 N‐labeled archaeal Rieske‐type [2Fe–2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high‐potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non‐coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high‐potential protein homologs, and may account for the tuning of Rieske‐type clusters in various redox systems.