Premium
Enhancement of angiogenic and vasculogenic potential of endothelial progenitor cells by haptoglobin
Author(s) -
Park Seon-Joo,
Baek Sang Hong,
Oh Mi-Kyung,
Choi Seung Hyun,
Park Eun Hye,
Kim Nam-Hoon,
Shin Jong-Chul,
Kim In-Sook
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.09.014
Subject(s) - matrigel , progenitor cell , endothelial progenitor cell , transfection , endothelial stem cell , chemistry , progenitor , in vivo , microbiology and biotechnology , angiogenesis , stem cell , biology , in vitro , cancer research , gene , biochemistry
Endothelial progenitor cells (EPCs) were transfected with the haptoglobin (Hp) gene to investigate the effect of Hp on cell function. Hp potentiated the gene expression of various pro‐angiogenic factors in the EPCs. The Hp‐modified EPCs also increased in vitro tube formation on Matrigel compared with control cells. In hindlimb ischaemia models, Hp–EPCs showed a greater ability for improving blood perfusion and recovery from ischaemic injury. These results indicate that Hp improves EPC function in neovasculogenesis, which suggests that ex vivo modification of EPCs with the Hp gene can be applied to the treatment of vascular damage.