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The transcription elongation factors NELF, DSIF and P‐TEFb control constitutive transcription in a gene‐specific manner
Author(s) -
Fujita Toshitsugu,
Piuz Isabelle,
Schlegel Werner
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.07.050
Subject(s) - transcription (linguistics) , p tefb , microbiology and biotechnology , rna polymerase ii , virology , gene , chemistry , biology , genetics , gene expression , promoter , philosophy , linguistics
We examined whether transcription elongation factors control constitutive transcription of the histoneH 1 ( 0 ) and GAPDH genes. Chromatin immunoprecipitaion demonstrated positive transcription elongation factor b (P‐TEFb) and 5,6‐dichloro‐1‐β‐ d ‐ribofuranosylbenzimidazole (DRB) sensitivity‐inducing factor (DSIF) present together with RNA polymerase II (pol II) throughout the histoneH 1 ( 0 ) gene, whereas negative elongation factor (NELF) was confined to the 5′ region. Contrarily, DSIF, NELF and pol II were confined to the 5′ region on the GAPDH . Inhibition of those factors affected the constitutive transcription of the histoneH 1 ( 0 ) gene but not the GAPDH gene. Thus, NELF, DSIF and P‐TEFb control constitutive transcription in a gene‐specific manner.