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Cholesterol reverts Triton X‐100 preferential solubilization of sphingomyelin over phosphatidylcholine: A 31 P‐NMR study
Author(s) -
Ahyayauch Hasna,
Collado M. Isabel,
Goñi Félix M.,
Lichtenberg Dov
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.07.046
Subject(s) - chemistry , micelle , phosphatidylcholine , solubilization , bilayer , sphingomyelin , triton x 100 , ternary operation , membrane , lamellar structure , octyl glucoside , vesicle , chromatography , crystallography , analytical chemistry (journal) , pulmonary surfactant , phospholipid , aqueous solution , biochemistry , computer science , programming language
The distribution of phosphatidylcholine (PC) and sphingomyelin (SM) between the solubilized (micellar) and non‐solubilized (lamellar) fractions arising from bilayers composed of PC and SM, with or without cholesterol (Chol) has been measured under conditions of partial, incomplete solubilization by Triton X‐100. Quantitation is achieved by 31 P‐NMR determination of the composition of mixed micelles in the range of bilayer‐micelle coexistence. We find that the solubilized fraction of bilayers consisting of binary mixtures of PC and SM is rich in SM, as expected from previous data on solubilization of pure PC and pure SM liposomes. In contrast, after partial solubilization of ternary mixtures of PC, SM and Chol, the solubilized fraction becomes SM‐poor, as observed in the partial solubilization of biomembranes.