Premium
Coordinated control of a designed trans ‐acting ligase ribozyme by a loop–receptor interaction
Author(s) -
Matsumura Shigeyoshi,
Ohmori Rei,
Saito Hirohide,
Ikawa Yoshiya,
Inoue Tan
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.07.036
Subject(s) - digital subscriber line , ribozyme , dna ligase , modular design , chemistry , loop (graph theory) , trans acting , ubiquitin ligase , biology , microbiology and biotechnology , computer science , biochemistry , rna , enzyme , ubiquitin , mathematics , operating system , computer network , combinatorics , gene , mutant
We previously developed a synthetic cis ‐acting RNA ligase ribozyme with 3′–5′ joining activity termed “DSL” (designed and selected ligase). DSL was easily transformed into a trans ‐acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans ‐acting DSL, t DSL‐1/GUAA. t DSL‐1/GUAA exhibited remarkably high activity compared with the parental cis ‐acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop–receptor interaction plays a significant role in determining the activity of the trans ‐acting ribozyme and in its ability to perform multiple turnovers of the reaction.