Coordinated control of a designed  trans ‐acting ligase ribozyme by a loop–receptor interaction | Zendy

Matsumura Shigeyoshi | Zendy; Ohmori Rei | Zendy; Saito Hirohide | Zendy; Ikawa Yoshiya | Zendy; Inoue Tan | Zendy

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Coordinated control of a designed trans ‐acting ligase ribozyme by a loop–receptor interaction

Author(s) -

Matsumura Shigeyoshi,

Ohmori Rei,

Saito Hirohide,

Ikawa Yoshiya,

Inoue Tan

Publication year - 2009

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2009.07.036

Subject(s) - digital subscriber line , ribozyme , dna ligase , modular design , loop (graph theory) , biology , chemistry , microbiology and biotechnology , computational biology , genetics , rna , biochemistry , computer science , mathematics , enzyme , programming language , computer network , combinatorics , gene

We previously developed a synthetic cis ‐acting RNA ligase ribozyme with 3′–5′ joining activity termed “DSL” (designed and selected ligase). DSL was easily transformed into a trans ‐acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans ‐acting DSL, t DSL‐1/GUAA. t DSL‐1/GUAA exhibited remarkably high activity compared with the parental cis ‐acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop–receptor interaction plays a significant role in determining the activity of the trans ‐acting ribozyme and in its ability to perform multiple turnovers of the reaction.

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