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RCAN1‐4 knockdown attenuates cell growth through the inhibition of Ras signaling
Author(s) -
Lee Hong Joon,
Kim Young Sun,
Sato Yasufumi,
Cho Young-Jin
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.07.023
Subject(s) - gene knockdown , cell growth , microbiology and biotechnology , signal transduction , chemistry , biology , biochemistry , apoptosis
Forced changes in the expression of regulator of calcineurin 1 (RCAN1) affects cell growth. This has been linked to the suppression of calcineurin‐nuclear factor of activated T cells signaling by RCAN1. Here, we describe a novel role of RCAN1 isoform 4 in proper expression of Ras protein and its signaling. RCAN1 isoform 4 knockdown attenuated growth factor‐induced extracellular signal‐regulated kinase activation and cell growth; reduced Ras levels and its translation rate; and led to a reduction of eukaryotic initiation factor 4E in the initiation complex and a slight repression of global protein synthesis. Experiments utilizing activity‐modified mutants of calcineurin A demonstrated that these effects were calcineurin‐independent. Our findings reveal a previously unknown role of RCAN1‐4 in protein synthesis, which may be relevant to cell growth.