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Intracellular ANKRD1 protein levels are regulated by 26S proteasome‐mediated degradation
Author(s) -
Badi Ileana,
Cinquetti Raffaella,
Frascoli Michela,
Parolini Cinzia,
Chiesa Giulia,
Taramelli Roberto,
Acquati Francesco
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.07.001
Subject(s) - intracellular , proteasome , microbiology and biotechnology , protein degradation , gene , chemistry , biology , biochemistry
The ANKRD1/CARP gene encodes a muscle‐specific protein which has been implicated in transcriptional regulation and myofibrillar assembly. Several features at both the mRNA and protein levels define ANKRD1 as a gene whose expression is tightly regulated, and deregulated expression of this protein has been recently associated to human congenital heart disease. It is therefore crucial to define the intracellular pathways that regulate the ANKRD1 protein's steady‐state levels. Here, we show that ANKRD1 is a short‐lived protein whose levels are tightly regulated by the 26S proteasome. In addition, a critical role for a putative PEST motif was established, although other degrons within the ANKRD1 protein are likely implicated in the control of its intracellular levels.