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3′‐Untranslated region of doublecortin mRNA is a binding target of the Musashi1 RNA‐binding protein
Author(s) -
Horisawa Kenichi,
Imai Takao,
Okano Hideyuki,
Yanagawa Hiroshi
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.06.045
Subject(s) - doublecortin , messenger rna , untranslated region , rna binding protein , translation (biology) , rna , microbiology and biotechnology , three prime untranslated region , chemistry , biology , gene , biochemistry , dentate gyrus , hippocampal formation , neuroscience
Musashi1 (Msi1) is an RNA‐binding protein that is highly expressed in neural stem cells, and is considered to be a stemness factor. A known function of Msi1 is translational repression of specifically bound mRNAs. Although the basic mechanism and some target RNAs have been reported, further survey of interactors is necessary to understand the integrated function of Msi1. By screening using an mRNA display technique, we found that doublecortin ( dcx ) mRNA is a specific binding target of Msi1 in vitro. We confirmed that Msil repressed translation of a luciferase reporter gene linked to the selected 3′‐untranslated region fragment of dcx in Neuro2A cells.