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Syndecan‐4 promotes the retention of phosphatidylinositol 4,5‐bisphosphate in the plasma membrane
Author(s) -
Kwon Soojin,
Son Hyowon,
Choi Youngsil,
Lee Jung-hyun,
Choi Sojoong,
Lim Yangmi,
Han Inn-Oc,
Oh Eok-Soo
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.06.039
Subject(s) - phosphatidylinositol 4,5 bisphosphate , phosphatidylinositol , syndecan 1 , phospholipase c , ionomycin , chemistry , microbiology and biotechnology , phospholipase , membrane , lipid bilayer fusion , biochemistry , kinase , biophysics , biology , signal transduction , intracellular , cell , enzyme
Although phosphatidylinositol 4,5‐bisphosphate (PIP 2 ) regulates syndecan‐4 function, the potential influence of syndecan‐4 on PIP 2 remains unknown. GFP containing PIP 2 ‐binding‐PH domain of phospholipase Cδ (GFP‐PHδ) was used to monitor PIP 2 . Syndecan‐4 overexpression in COS‐7 cells enhanced membrane translocation of GFP‐PHδ, while the opposite was observed when syndecan‐4 was knocked‐down. PIP 2 levels were higher in total phospholipids extracted from rat embryo fibroblasts expressing syndecan‐4. Syndecan‐4‐induced membrane targeting of GFP‐PHδ was further enhanced by phosphoinositide‐3‐kinase inhibitor, but not by phospholipase C (PLC) inhibitor. Besides, both ionomycin and epidermal growth factor caused dissociation of GFP‐PHδ from plasma membrane, an effect that was significantly delayed by syndecan‐4 over‐expression. Collectively, these data suggest that syndecan‐4 promotes plasma membrane retention of PIP 2 by negatively regulating PLC‐dependent PIP 2 degradation.