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Effects of the BH3‐only protein human Noxa on mitochondrial dynamics
Author(s) -
Woo Ha-Na,
Seo Young-Woo,
Moon Ae Ran,
Jeong Seon-Yong,
Jeong Seong-Yun,
Choi Eun Kyung,
Kim Tae-Hyoung
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.06.029
Subject(s) - mitochondrion , bcl 2 family , fragmentation (computing) , microbiology and biotechnology , apoptosis , biology , chemistry , programmed cell death , biochemistry , ecology
Mitochondria form reticular networks comprised of filamentous tubules and continuously move and change shape. Bcl‐2 family proteins actively participate in the regulation of mitochondria fragmentation. Here, we show that human Noxa, which belongs to the BH3‐only pro‐apoptotic Bcl‐2 family, causes mitochondrial fragmentation. We found that while the Bcl‐2 homology 3 (BH3) domain of Noxa is not associated with mitochondrial fragmentation, the mitochondrial targeting domain (MTD) of Noxa is the region responsible for inducing fragmentation. Two leucine residues in MTD play a key role in the process. Furthermore, the lack of Noxa causes a significant reduction of Velcade‐induced mitochondrial fragmentation. Together, these results provide novel insight into the role of Noxa in mitochondrial dynamics and cell death.