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NAD(P)H‐ and superoxide‐dependent nitric oxide degradation by rat liver mitochondria
Author(s) -
Oliveira Halley Caixeta,
Saviani Elzira Elisabeth,
Salgado Ione
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.06.012
Subject(s) - nad+ kinase , mitochondrion , superoxide , nitric oxide , respiratory chain , chemistry , biochemistry , electron transport chain , biophysics , enzyme , biology , organic chemistry
Mitochondria consume nitric oxide (NO) mainly through reaction with superoxide anion (O 2 ‐). Here, we analyzed theO 2 ‐sources for NO degradation by isolated rat liver mitochondria. Electron leakage from complex III and reverse electron transport to complex I accounted forO 2 ‐‐dependent NO degradation by mitochondria in the presence of a protonmotive force. Mitochondria incubated with NAD(P)H also presented intenseO 2 ‐generation and NO degradation rates that were insensitive to respiratory inhibitors and abolished after proteinase treatment. These results suggest that an outer membrane‐localized NAD(P)H oxidase activity, in addition to the electron leakage from the respiratory chain, promotesO 2 ‐‐dependent NO degradation in rat liver mitochondria.