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miR‐210 promotes osteoblastic differentiation through inhibition of AcvR1b
Author(s) -
Mizuno Yosuke,
Tokuzawa Yoshimi,
Ninomiya Yuichi,
Yagi Ken,
Yatsuka-Kanesaki Yukiko,
Suda Tatsuo,
Fukuda Toru,
Katagiri Takenobu,
Kondoh Yasumitsu,
Amemiya Tomoyuki,
Tashiro Hideo,
Okazaki Yasushi
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.06.006
Subject(s) - microbiology and biotechnology , transfection , stromal cell , transforming growth factor , microrna , chemistry , cellular differentiation , signal transduction , osteoblast , regulator , receptor , cancer research , biology , gene , in vitro , biochemistry
Although microRNAs (miRNAs) are involved in many biological processes, the mechanisms whereby miRNAs regulate osteoblastic differentiation are poorly understood. Here, we found that BMP‐4‐induced osteoblastic differentiation of bone marrow‐derived ST2 stromal cells was promoted and repressed after transfection of sense and antisense miR‐210, respectively. A reporter assay demonstrated that the activin A receptor type 1B ( AcvR1b ) gene was a target for miR‐210. Furthermore, inhibition of transforming growth factor‐β (TGF‐β)/activin signaling in ST2 cells with SB431542 promoted osteoblastic differentiation. We conclude that miR‐210 acts as a positive regulator of osteoblastic differentiation by inhibiting the TGF‐β/activin signaling pathway through inhibition of AcvR1b .