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Subcellular localization of rice histone deacetylases in organelles
Author(s) -
Chung Pil Joong,
Kim Youn Shic,
Park Su-Hyun,
Nahm Baek Hie,
Kim Ju-Kon
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.06.003
Subject(s) - green fluorescent protein , chloroplast , arabidopsis , mitochondrion , subcellular localization , organelle , microbiology and biotechnology , histone , transgene , biology , chemistry , biochemistry , gene , cytoplasm , mutant
Histone deacetylases (HDACs) are known to function in the nucleus. Here, we report on the organellar localization of three rice HDACs, OsSIR2b, OsHDAC6, and OsHDAC10. The 35S:OsSIR2b‐GFP and 35S:OsHDAC10‐GFP constructs were introduced into tobacco BY2 cells. Co‐localization analysis of the green fluorescent protein and MitoTracker fluorescent signals in the transformed BY2 cells indicated that OsSIR2b and OsHDAC10 are localized in the mitochondria. Transgenic Arabidopsis lines harboring 35S:OsHDAC6‐GFP and 35S:OsHDAC10‐GFP constructs were similarly analyzed, revealing that OsHDAC6‐GFP is localized exclusively in chloroplasts, whereas OsHDAC10‐GFP is localized in both mitochondria and chloroplasts. The presence of OsHDAC6‐GFP and OsHDAC10‐GFP in chloroplasts was verified by immunodetection.