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A nucleolar protein RRS1 contributes to chromosome congression
Author(s) -
Gambe Arni E.,
Matsunaga Sachihiro,
Takata Hideaki,
Ono-Maniwa Rika,
Baba Akiko,
Uchiyama Susumu,
Fukui Kiichi
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.033
Subject(s) - microbiology and biotechnology , mitosis , nucleolus , sister chromatids , interphase , biology , separase , chromosome segregation , chromosome , genetics , gene , cytoplasm
We report here the functional analysis of human Regulator of Ribosome Synthesis 1 (RRS1) protein during mitosis. We demonstrate that RRS1 localizes in the nucleolus during interphase and is distributed at the chromosome periphery during mitosis. RNA interference experiments revealed that RRS1‐depleted cells show abnormalities in chromosome alignment and spindle organization, which result in mitotic delay. RRS1 knockdown also perturbs the centromeric localization of Shugoshin 1 and results in premature separation of sister chromatids. Our results suggest that a nucleolar protein RRS1 contributes to chromosome congression.