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House dust mite allergen Der f 1 can induce the activation of latent TGF‐β via its protease activity
Author(s) -
Nakamura Yuki,
Miyata Masanori,
Shimokawa Naomi,
Ohnuma Yuko,
Katoh Ryohei,
Ogawa Hideoki,
Okumura Ko,
Nakao Atsuhito
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.030
Subject(s) - protease , proteases , chemistry , house dust mite , cleave , transforming growth factor , cysteine protease , allergen , papain , biochemistry , microbiology and biotechnology , biology , enzyme , immunology , allergy
A major house dust mite allergen Der f 1 belongs to the papain‐like cysteine protease family. This study investigated whether Der f 1 can cleave the latency‐associated peptide (LAP) of transforming growth factor (TGF)‐β via its proteolytic activity and activate latent TGF‐β. We found that Der f 1 can cleave LAP and induce the activation of latent TGF‐β, leading to functional Smad signaling. Importantly, these actions of Der f 1 were inhibited by cysteine protease inhibitor E64 or inactivation of the protease activity by heat. Thus, latent TGF‐β may be a direct target of Der f 1 protease activity.