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Differential changes in GPR55 during microglial cell activation
Author(s) -
Pietr Maciej,
Kozela Ewa,
Levy Rivka,
Rimmerman Neta,
Lin Yi Hsing,
Stella Nephi,
Vogel Zvi,
Juknat Ana
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.028
Subject(s) - microglia , neuroinflammation , microbiology and biotechnology , cannabinoid receptor , receptor , cannabinoid , agonist , chemistry , mapk/erk pathway , lipopolysaccharide , biology , phosphorylation , endocrinology , immunology , inflammation , biochemistry
We examined how lipopolysaccharide (LPS) and interferon gamma (IFN‐γ), known to differentially activate microglia, affect the expression of G protein‐coupled receptor 55 (GPR55), a novel cannabinoid receptor. We found that GPR55 mRNA is significantly expressed in both primary mouse microglia and the BV‐2 mouse microglial cell line, and that LPS down‐regulates this message. Conversely, IFN‐γ slightly decreases GPR55 mRNA in primary microglia, while it upregulates this message in BV‐2 cells. Moreover, the GPR55 agonist, lysophosphatidylinositol, increases ERK phosphorylation in BV‐2 stimulated with IFN‐γ, in correlation with the increased amount of GPR55 mRNA. Remarkably, these stimuli‐induced changes in GPR55 expression are similar to those observed with CB 2 ‐R, suggesting that both receptors might be involved in neuroinflammation and that their expression is concomitantly controlled by the state of microglial activation.