Posttranscriptional down‐regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency | Zendy

Badhai Jitendra | Zendy; Fröjmark Anne-Sophie | Zendy; Razzaghian Hamid Reza | Zendy; Davey Edward | Zendy; Schuster Jens | Zendy; Dahl Niklas | Zendy

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Posttranscriptional down‐regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency

Author(s) -

Badhai Jitendra,

Fröjmark Anne-Sophie,

Razzaghian Hamid Reza,

Davey Edward,

Schuster Jens,

Dahl Niklas

Publication year - 2009

Publication title -

febs letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.593

H-Index - 257

eISSN - 1873-3468

pISSN - 0014-5793

DOI - 10.1016/j.febslet.2009.05.023

Subject(s) - ribosomal protein , ribosomal rna , protein subunit , eukaryotic large ribosomal subunit , eukaryotic small ribosomal subunit , 23s ribosomal rna , rna , microbiology and biotechnology , biology , 18s ribosomal rna , ribosome , biochemistry , gene

Ribosomal protein S19 (RPS19) is mutated in patients with Diamond‐Blackfan anemia (DBA). We hypothesized that decreased levels of RPS19 lead to a coordinated down‐regulation of other ribosomal (r‐)proteins at the subunit level. We show that small interfering RNA (siRNA) knock‐down of RPS19 results in a relative decrease of small subunit (SSU) r‐proteins (S20, S21 and S24) when compared to large subunit (LSU) r‐proteins (L3, L9, L30 and L38). This correlates with a relative decrease in 18S rRNA with respect to 28S rRNA. The r‐protein mRNA levels remain relatively unchanged indicating a post transcriptional regulation of r‐proteins at the level of subunit formation.

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