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Posttranscriptional down‐regulation of small ribosomal subunit proteins correlates with reduction of 18S rRNA in RPS19 deficiency
Author(s) -
Badhai Jitendra,
Fröjmark Anne-Sophie,
Razzaghian Hamid Reza,
Davey Edward,
Schuster Jens,
Dahl Niklas
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.023
Subject(s) - ribosomal protein , ribosomal rna , protein subunit , eukaryotic large ribosomal subunit , eukaryotic small ribosomal subunit , 23s ribosomal rna , rna , microbiology and biotechnology , biology , 18s ribosomal rna , ribosome , biochemistry , gene
Ribosomal protein S19 (RPS19) is mutated in patients with Diamond‐Blackfan anemia (DBA). We hypothesized that decreased levels of RPS19 lead to a coordinated down‐regulation of other ribosomal (r‐)proteins at the subunit level. We show that small interfering RNA (siRNA) knock‐down of RPS19 results in a relative decrease of small subunit (SSU) r‐proteins (S20, S21 and S24) when compared to large subunit (LSU) r‐proteins (L3, L9, L30 and L38). This correlates with a relative decrease in 18S rRNA with respect to 28S rRNA. The r‐protein mRNA levels remain relatively unchanged indicating a post transcriptional regulation of r‐proteins at the level of subunit formation.