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Inhibition of adipocyte differentiation by RORα
Author(s) -
Duez Hélène,
Duhem Christian,
Laitinen Saara,
Patole Prashant S.,
Abdelkarim Mouaadh,
Bois-Joyeux Brigitte,
Danan Jean-Louis,
Staels Bart
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.019
Subject(s) - adipogenesis , ectopic expression , adipocyte , gene isoform , adipose tissue , phenotype , embryonic stem cell , biology , microbiology and biotechnology , orphan receptor , nuclear receptor , medicine , endocrinology , 3t3 l1 , gene , gene expression , chemistry , transcription factor , biochemistry
Here we show that gene expression of the nuclear receptor RORα is induced during adipogenesis, with RORα4 being the most abundantly expressed isoform in human and murine adipose tissue. Over‐expression of RORα4 in 3T3‐L1 cells impairs adipogenesis as shown by the decreased expression of adipogenic markers and lipid accumulation, accompanied by decreased free fatty acid and glucose uptake. By contrast, mouse embryonic fibroblasts from staggerer mice, which carry a mutation in the RORα gene, differentiate more efficiently into mature adipocytes compared to wild‐type cells, a phenotype which is reversed by ectopic RORα4 restoration.