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An anticoagulant peptide from the human hookworm, Ancylostoma duodenale that inhibits coagulation factors Xa and XIa
Author(s) -
Gan Weiqiong,
Deng Li,
Yang Chen,
He Qingfeng,
Hu Jingjing,
Yin Huan,
Jin Xian,
Lu Chengyu,
Wu Yamin,
Peng Lifei
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.009
Subject(s) - ancylostoma , ancylostoma duodenale , partial thromboplastin time , peptide , biology , biochemistry , immunology , necator americanus , platelet , helminths , ascaris lumbricoides
A full‐length cDNA encoding an anticoagulant peptide, named AduNAP4, was cloned and identified from the human hookworm Ancylostoma duodenale . AduNAP4 has 104 amino acids including a predicted 23‐residue signal peptide and shows ⩽50% similarity with other known nematode anticoagulant protein/peptide (NAP). AduNAP4 is extremely efficient at prolonging the activated partial thromboplastin time, and is an inhibitor of both fXa ( K i = 7.34 ± 1.74 nM) and fXIa ( K i = 42.45 ± 3.25 nM). No fXIa inhibitor has previously been described from other blood‐feeding animals. Our results suggest that hookworms have evolved a potent mechanism that interferes with coagulation by inhibition of fXIa to facilitate its blood‐feeding lifestyle.