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Arabidopsis thaliana expresses a second functional flavonol synthase
Author(s) -
Preuß Anja,
Stracke Ralf,
Weisshaar Bernd,
Hillebrecht Alexander,
Matern Ulrich,
Martens Stefan
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.05.006
Subject(s) - flavonols , arabidopsis , arabidopsis thaliana , anthocyanidins , mutant , biochemistry , flavonoid , flavonoid biosynthesis , chemistry , biology , enzyme , gene , gene expression , transcriptome , antioxidant
Arabidopsis thaliana L. produces flavonoid pigments, i.e. flavonols, anthocyanidins and proanthocyanidins, from dihydroflavonol substrates. A small family of putative flavonol synthase (FLS) genes had been recognized in Arabidopsis , and functional activity was attributed only to FLS1. Nevertheless, other FLS activities must be present, because A. thaliana fls1 mutants still accumulate significant amounts of flavonols. The recombinant FLSs and leucoanthocyanidin dioxygenase (LDOX) proteins were therefore examined for their enzyme activities, which led to the identification of FLS3 as a second active FLS. This enzyme is therefore likely responsible for the formation of flavonols in the ldox/fls1‐2 double mutant. These double mutant and biochemical data demonstrate for the first time that LDOX is capable of catalyzing the in planta formation of flavonols.