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Molecular and functional characterization of adipokinetic hormone receptor and its peptide ligands in Bombyx mori
Author(s) -
Zhu Chenggang,
Huang Haishan,
Hua Rongsheng,
Li Guo,
Yang Dong,
Luo Jiansong,
Zhang Cunxin,
Shi Liangen,
Benovic Jeffrey L.,
Zhou Naiming
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.060
Subject(s) - adipokinetic hormone , bombyx mori , hek 293 cells , receptor , stimulation , intracellular , biochemistry , signal transduction , extracellular , microbiology and biotechnology , peptide , chemistry , kinase , hormone , biology , neuropeptide , endocrinology , gene
Neuropeptides of the adipokinetic hormone (AKH) family are among the best studied hormone peptides, but its signaling pathways remain to be elucidated. In this study, we molecularly characterized the signaling of Bombyx AKH receptor (AKHR) and its peptide ligands in HEK293 cells. In HEK293 cells stably expressing AKHR, AKH1 stimulation not only led to a ligand concentration dependent mobilization of intracellular Ca 2+ and cAMP accumulation, but also elicited transient activation of extracellular signal‐regulated kinase 1/2 (ERK1/2) pathway. We observed that AKH receptor was rapidly internalized after AKH1 stimulation. We further demonstrated that AKH2 exhibited high activities in cAMP accumulation and ERK1/2 activation on AKHR comparable to AKH1, whereas AKH3 was much less effective.