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Fluorescence microscopy studies on islet amyloid polypeptide fibrillation at heterogeneous and cellular membrane interfaces and its inhibition by resveratrol
Author(s) -
Radovan Diana,
Opitz Norbert,
Winter Roland
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.059
Subject(s) - islet , chemistry , amyloid (mycology) , fluorescence microscope , fibril , biochemistry , biophysics , lipid raft , membrane , lipid bilayer , microbiology and biotechnology , fluorescence , insulin , biology , endocrinology , inorganic chemistry , physics , quantum mechanics
Type II diabetes mellitus (T2DM) is a disease characterized by progressive deposition of amyloid in the extracellular matrix of β‐cells. We investigated the interaction of the islet amyloid polypeptide (IAPP) with lipid model raft mixtures and INS‐1E cells using fluorescence microscopy techniques. Following preferential partitioning of IAPP into the fluid lipid phase, the membrane suffers irreversible damage and predominantly circularly‐shaped lipid‐containing IAPP amyloid is formed. Interaction studies with the pancreatic β‐cell line INS‐1E revealed that growing IAPP fibrils also incorporate substantial amounts of cellular membranes in vivo. Additionally, the inhibitory effect of the red wine compound resveratrol on IAPP fibril formation has been studied, alluding to its potential use in developing therapeutic strategies against T2DM.