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Solution structure of DnaE intein from Nostoc punctiforme : Structural basis for the design of a new split intein suitable for site‐specific chemical modification
Author(s) -
Oeemig Jesper S.,
Aranko A. Sesilja,
Djupsjöbacka Janica,
Heinämäki Kimmo,
Iwaï Hideo
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.058
Subject(s) - intein , trans splicing , protein splicing , rna splicing , chemistry , biochemistry , gene , rna
Naturally split DnaE intein from Nostoc punctiforme ( Npu ) has robust protein trans ‐splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of Npu DnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain Npu DnaE intein, we designed a functional split variant of the Npu DnaE intein having a short C‐terminal half (C‐intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated.