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The C‐terminal cytoplasmic domain of human proEGF is a negative modulator of body and organ weights in transgenic mice
Author(s) -
Klonisch Thomas,
Glogowska Aleksandra,
Gratao Ana A.,
Grzech Marjeta,
Nistor Andreea,
Torchia Mark,
Weber Ekkehard,
de Angelis Martin Hrabé,
Rathkolb Birgit,
Hoang-Vu Cuong,
Wolf Eckhard,
Schneider Marlon R.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.051
Subject(s) - genetically modified mouse , transgene , cytoplasm , biology , kidney , in vivo , histology , endocrinology , medicine , gene , microbiology and biotechnology , biochemistry , genetics
We generated transgenic mice to study the in vivo role of the cytoplasmic domain of human proEGF (proEGFcyt). Post‐pubertal proEGFcyt transgenic (tg) mice displayed an up to 15% reduction in body weight, including smaller kidney and brain weights as compared to control littermates. Renal histology, gene expression profiles, and functional parameters were normal. In both sexes, serum levels of IGFBP‐3 were reduced. Circulating IGF‐I/IGF‐II levels were unchanged. Histomorphological analysis revealed isolated foci of liver necrosis specific to proEGFcyt tg mice. In conclusion, we identified proEGF cytoplasmic domain as a novel modulator of whole body and organ‐specific growth in mice.