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Functional cloning of genes that suppress oxidative stress‐induced cell death: TCTP prevents hydrogen peroxide‐induced cell death
Author(s) -
Nagano-Ito Michiyo,
Banba Aoi,
Ichikawa Shinichi
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.045
Subject(s) - oxidative stress , microbiology and biotechnology , cloning (programming) , complementary dna , programmed cell death , gene , apoptosis , cdna library , cell culture , biology , chemistry , biochemistry , genetics , computer science , programming language
We used retroviral‐mediated expression cloning to identify cDNAs that inhibit cell death induced by oxidative stress. To isolate the genes, we introduced a murine embryonic retroviral cDNA library into NIH/3T3 cells, and selected for cells resistant to hydrogen peroxide. The surviving cells were cloned, and the integrated cDNAs were rescued by polymerase chain reaction. Several of the isolated cDNAs are known to be involved in modulating the redox state of cells. Other cDNAs encode proteins known to suppress apoptosis caused by reasons other than oxidative stress. These included polyadenylate‐binding protein, cytosolic 1 (Pabpc1) and translationally controlled tumor protein (TCTP).