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Focal adhesion disassembly requires clathrin‐dependent endocytosis of integrins
Author(s) -
Chao Wei-Ting,
Kunz Jeannette
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.037
Subject(s) - dynamin , endocytosis , clathrin , microbiology and biotechnology , integrin , internalization , focal adhesion , cell adhesion , adhesion , receptor mediated endocytosis , chemistry , biology , cell , signal transduction , biochemistry , organic chemistry
Cell migration requires the controlled disassembly of focal adhesions, but the underlying mechanisms remain poorly understood. Here, we show that adhesion turnover is mediated through dynamin‐ and clathrin‐dependent endocytosis of activated β1 integrins. Consistent with this, clathrin and the clathrin adaptors AP‐2 and disabled‐2 (DAB2) distribute along with dynamin 2 to adhesion sites prior to adhesion disassembly. Moreover, knockdown of either dynamin 2 or both clathrin adaptors blocks β1 integrin internalization, leading to impaired focal adhesion disassembly and cell migration. Together, these results provide important insight into the mechanisms underlying adhesion disassembly and identify novel components of the disassembly pathway.