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Profiling CD antigens on leukaemias with an antibody microarray
Author(s) -
Barber Nicole,
Gez Swetlana,
Belov Larissa,
Mulligan Stephen P.,
Woolfson Adrian,
Christopherson Richard I.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.018
Subject(s) - antigen , monoclonal antibody , flow cytometry , antibody , microbiology and biotechnology , antibody microarray , cluster of differentiation , biology , microarray , dna microarray , cancer research , chemistry , immunology , gene , cell , gene expression , genetics
Cluster of differentiation (CD) antigens are defined when a surface molecule found on some members of a standard panel of human cells reacts with at least one novel antibody, and there is good accompanying molecular data. Monoclonal antibodies to surface CD antigens on leukocytes have been used for flow cytometry, and more recently to construct microarrays that capture live cells. These DotScan TM microarrays enable the rapid and highly parallel characterization of repertoires of CD antigens whose expression patterns may be correlated with discrete leukaemia subtypes, or used to define biomarker ‘signatures’ for non‐hematological diseases. DotScan TM with fluorescence multiplexing enables profiling of CD antigens for minor subsets of cells, such as colorectal cancer cells and tumour‐infiltrating lymphocytes from a surgical sample.