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Brefeldin A inhibits expression of DNA packaging proteins and nucleocapsid formation of human cytomegalovirus
Author(s) -
Kregler Oliver,
Schilf Rita,
Lander Angelika,
Bannert Norbert,
Bogner Elke
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.011
Subject(s) - brefeldin a , viral replication , capsid , microbiology and biotechnology , dna , biology , dna replication , virology , human cytomegalovirus , chemistry , virus , golgi apparatus , biochemistry , endoplasmic reticulum
In this study we used the fungal antibiotic brefeldin A (BFA) to analyze its effect on viral replication. Analysis by electron microscopy demonstrated that no viral particles were observed in cells treated before the onset of viral replication. In the presence of BFA expression of IE2, MCP, pUL104, pUL56 and pUL89 were reduced, while no or slight effect was observed on expression of pp65, pUL44 and pUL57. Strikingly, real time PCR revealed that de novo viral DNA synthesis is reduced but not completely abolished in the presence of BFA. These results indicated that BFA represents a multi‐functional compound leading to inhibition of several steps of viral maturation such as expression of viral DNA packaging proteins and capsid formation.