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SIRT1 regulates tyrosine hydroxylase expression and differentiation of neuroblastoma cells via FOXO3a
Author(s) -
Kim Min-Ju,
Ahn Kyungsook,
Park Seong-Hoon,
Kang Hong-Jun,
Jang Bong Geom,
Oh Soo-Jin,
Oh Sun-Mee,
Jeong Yu-Jin,
Heo Jee-In,
Suh Jun-Gyo,
Lim Soon Sung,
Ko Yoon-Jung,
Huh Sung-Oh,
Kim Sung Chan,
Park Jae-Bong,
Kim Jaebong,
Kim Jong-Il,
Jo Sangmee Ahn,
Lee Jae-Yong
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.007
Subject(s) - nicotinamide , tyrosine hydroxylase , retinoic acid , neuroblastoma , histone deacetylase , acetylation , cellular differentiation , neurite , chemistry , histone , microbiology and biotechnology , cancer research , biology , biochemistry , cell culture , enzyme , in vitro , gene , genetics
To examine the function of SIRT1 in neuronal differentiation, we employed all‐trans retinoic acid (ATRA)‐induced differentiation of neuroblastoma cells. Nicotinamide inhibited neurite outgrowth and tyrosine hydroxylase (TH) expression. Inhibition of PARP or histone deacetylase did not inhibit TH expression, showing the effect to be SIRT1 specific. Expression of FOXO3a and its target proteins were increased during the differentiation and reduced by nicotinamide. FOXO3a deacetylation was increased by ATRA and blocked by nicotinamide. SIRT1 and FOXO3a siRNA inhibited ATRA‐induced up‐regulation of TH and differentiation. Taken together, these results indicate that SIRT1 is involved in ATRA‐induced differentiation of neuroblastoma cells via FOXO3a.