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Protein complementation assays: Approaches for the in vivo analysis of protein interactions
Author(s) -
Morell Montse,
Ventura Salvador,
Avilés Francesc X.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.03.002
Subject(s) - protein fragment complementation assay , in vivo , computational biology , complementation , protein–protein interaction , identification (biology) , bimolecular fluorescence complementation , drug discovery , chemistry , biochemistry , biology , phenotype , genetics , gene , botany
The in vivo identification and characterization of protein–protein interactions (PPIs) are essential to understand cellular events in living organisms. In this review, we focus on protein complementation assays (PCAs) that have been developed to detect in vivo protein interactions as well as their modulation or spatial and temporal changes. The uses of PCAs are increasing, spanning different areas such as the study of biochemical networks, screening for protein inhibitors and determination of drug effects. Emphasis is given to approaches that rely on signals of spectroscopic nature (i.e. fluorescence or luminescence), the ones that are more directly related to bioimaging.