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Stochastically determined directed movement explains the dominant small‐scale mitochondrial movements within non‐neuronal tissue culture cells
Author(s) -
Saunter Christopher D.,
Perng Ming Der,
Love Gordon D.,
Quinlan Roy A.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.02.041
Subject(s) - movement (music) , biological system , brownian motion , tracking (education) , confocal , physics , spinning , biology , diffusion , phase (matter) , confocal microscopy , biophysics , chemistry , optics , acoustics , psychology , pedagogy , quantum mechanics , polymer chemistry , thermodynamics
The apparently stationary phase of mitochondrial motion was investigated in epithelial cells by spinning disk confocal light microscopy combined with image correlation based single particle tracking using custom software producing sub‐pixel accuracy measurements (∼5 nm) at 10–12 Hz frame‐rates. The analysis of these data suggests that the previously described stationary, or anchored phase, in mitochondrial movement actually comprise Brownian diffusion, interspersed with frequent and brief motor‐driven events whose duration are stochastically determined. We have therefore discovered a new aspect of mitochondrial behavior, which we call stochastically determined, directed movement.