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Adipogenic differentiation of human induced pluripotent stem cells: Comparison with that of human embryonic stem cells
Author(s) -
Taura Daisuke,
Noguchi Michio,
Sone Masakatsu,
Hosoda Kiminori,
Mori Eisaku,
Okada Yohei,
Takahashi Kazutoshi,
Homma Koichiro,
Oyamada Naofumi,
Inuzuka Megumi,
Sonoyama Takuhiro,
Ebihara Ken,
Tamura Naohisa,
Itoh Hiroshi,
Suemori Hirofumi,
Nakatsuji Norio,
Okano Hideyuki,
Yamanaka Shinya,
Nakao Kazuwa
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.02.031
Subject(s) - embryoid body , adipogenesis , induced pluripotent stem cell , embryonic stem cell , microbiology and biotechnology , stem cell , cellular differentiation , kosr , biology , cell culture , chemistry , biochemistry , genetics , mesenchymal stem cell , gene
Induced pluripotent stem (iPS) cells were recently established from human fibroblasts. In the present study we investigated the adipogenic differentiation properties of four human iPS cell lines and compared them with those of two human embryonic stem (ES) cell lines. After 12 days of embryoid body formation and an additional 10 days of differentiation on Poly‐ l ‐ornithine and fibronectin‐ coated dishes with adipogenic differentiation medium, human iPS cells exhibited lipid accumulation and transcription of adipogenesis‐related molecules such as C/EBPα, PPARγ2, leptin and aP2. These results demonstrate that human iPS cells have an adipogenic potential comparable to human ES cells.