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SV channels dominate the vacuolar Ca 2+ release during intracellular signaling
Author(s) -
Pottosin Igor,
Wherrett Tim,
Shabala Sergey
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.02.009
Subject(s) - calmodulin , cytosol , calcium , vacuole , biophysics , signal transduction , chemistry , calcium signaling , intracellular , microbiology and biotechnology , second messenger system , patch clamp , biochemistry , biology , receptor , cytoplasm , enzyme , organic chemistry
Vacuoles have long been suggested to mediate a rise in the cytosolic free Ca 2+ during environmental signal transduction. This study addresses the issue of the control of vacuolar calcium release by some of the known signaling molecules such as IP 3 , cADPR, ABA, ATP, cAMP, cGMP, H 2 O 2 and CaM. Over 30 concentrations and/or combinations of these signaling compounds were studied in a series of electrophysiological experiments involving non‐invasive ion flux measurements (the MIFE) and patch‐clamp techniques. Our results suggest that calcium, calmodulin and nucleotides cause calcium release via SV channels.