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Release of extracellular membrane vesicles from microvilli of epithelial cells is enhanced by depleting membrane cholesterol
Author(s) -
Marzesco Anne-Marie,
Wilsch-Bräuninger Michaela,
Dubreuil Véronique,
Janich Peggy,
Langenfeld Katja,
Thiele Christoph,
Huttner Wieland B.,
Corbeil Denis
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.01.048
Subject(s) - vesicle , microbiology and biotechnology , membrane , chemistry , cell membrane , secretory vesicle , ultrastructure , cholesterol , biophysics , biochemistry , biology , anatomy
We previously reported on the occurrence of prominin‐1‐carrying membrane vesicles that are released into body fluids from microvilli of epithelial cells. This release has been implicated in cell differentiation. Here we have characterized these vesicles released from the differentiated Caco‐2 cells. We find that in these vesicles, prominin‐1 directly interacts with membrane cholesterol and is associated with a membrane microdomain. The cholesterol depletion using methyl‐β‐cyclodextrin resulted in a marked increase in their release, and a dramatic change in the microvillar ultrastructure from a tubular shape to a “pearling” state, with multiple membrane constrictions, suggesting a role of membrane cholesterol in vesicle release from microvilli.