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The immunostimulatory effect of IL‐1β in vivo is blocked by antisense peptides complementary to the loop sequence 163–171
Author(s) -
Boraschi Diana,
Tagliabue Aldo,
Miller Andrew D.
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.01.038
Subject(s) - in vivo , loop (graph theory) , peptide , in vitro , cytokine , chemistry , microbiology and biotechnology , biology , biochemistry , immunology , genetics , mathematics , combinatorics
Antisense (AS) peptides complementary to the β‐bulge surface loop VQGEESNDK (Boraschi loop) of the cytokine interleukin‐1β (IL‐1β) have been shown to bind IL‐1β at the Boraschi loop position, and to inhibit some of the IL‐1β‐mediated biological effects in vitro . Here we show that primary AS peptide FVITFFSLY inhibits IL‐1β‐mediated immunostimulation in vivo in a dose‐dependent fashion, while inactive on IL‐1β‐induced inflammation, an effect that takes place independently of the Boraschi loop. To the best of our knowledge, this is the first time that an AS peptide has been used successfully in vivo .