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Functional analysis of spfD gene involved in DNA phosphorothioation in Pseudomonas fluorescens Pf0‐1
Author(s) -
Yao Fen,
Xu Tiegang,
Zhou Xiufen,
Deng Zixin,
You Delin
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.01.029
Subject(s) - pseudomonas fluorescens , gene , gene cluster , dna , biology , homology (biology) , pseudomonas , atpase , genetics , microbiology and biotechnology , chemistry , biochemistry , bacteria , enzyme
DNA phosphorothioation is widespread in many bacterial species. By homology analysis of the dnd gene cluster in Pseudomonas fluorescens Pf0‐1, a spfBCDE gene cluster involved in DNA phosphorothioation was localized. Disruption of the spfD gene, a dndD homolog, caused the loss of the Dnd phenotype and demonstrated the involvement of spf D in DNA phosphorothioation in P. fluorescens Pf0‐1. The ATPase activity of SpfD suggests that SpfD could hydrolyze ATP to provide the energy required in the DNA phosphorothioate modification process.