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Hypotonic shocks activate rat TRPV4 in yeast in the absence of polyunsaturated fatty acids
Author(s) -
Loukin Stephen H.,
Su Zhenwei,
Kung Ching
Publication year - 2009
Publication title -
febs letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.593
H-Index - 257
eISSN - 1873-3468
pISSN - 0014-5793
DOI - 10.1016/j.febslet.2009.01.027
Subject(s) - trpv4 , transient receptor potential channel , yeast , polyunsaturated fatty acid , chemistry , trpv1 , biochemistry , electroporation , enzyme , microbiology and biotechnology , fatty acid , receptor , biology , gene
Transient‐receptor‐potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca 2+ rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks activate TRPV4 but not TRPV1. Osmotic swelling is modeled to activate enzyme(s), producing polyunsaturated fatty acids (PUFAs) to open TRPV4 in mammalian cells. This model relegates mechanosensitivity to the enzyme and not the channel. Yeast has only a single Δ9 fatty‐acid monodesaturase and cannot make PUFAs suggesting an alternative mechanism for TRPV4 activation. We discuss possible explanations of this difference.